Nanoscale resolution in GFP-based microscopy
نویسندگان
چکیده
منابع مشابه
Concepts for nanoscale resolution in fluorescence microscopy.
Spatio-temporal visualization of cellular structures by fluorescence microscopy has become indispensable in biology. However, the resolution of conventional fluorescence microscopy is limited by diffraction to about 180 nm in the focal plane and to about 500 nm along the optic axis. Recently, concepts have emerged that overcome the diffraction resolution barrier fundamentally. Formed on the bas...
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Kristin A. Gabor,1,2,3 Mudalige S. Gunewardene,1 David Santucci,4 and Samuel T. Hess1,3,* 1 Department of Physics and Astronomy, 120 Bennett Hall, University of Maine, Orono, ME 04469 2 Department of Molecular and Biomedical Sciences, 5735 Hitchner Hall, University of Maine, Orono, ME 04469 3 Graduate School of Biomedical Sciences, 263 ESRB/Barrows Hall, University of Maine, Orono, ME 04469 4 V...
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In collaboration with GSI Biophysics, the microbeam, operated with ions from the UNILAC linear accelerator of GSI, is routinely employed for targeted irradiation of living cells in culture [1]. A custom-made fluorescence microscope is used both for beam targeting and for cell observation via fluorescent dyes that specifically highlight the biological agent of interest. Green Fluorescent Protein...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2006
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth922